Inhibition of human papillomavirus replication by using artificial zinc-finger nucleases
نویسندگان
چکیده
منابع مشابه
Inhibition of virus DNA replication by artificial zinc finger proteins.
Prevention of virus infections is a major objective in agriculture and human health. One attractive approach to the prevention is inhibition of virus replication. To demonstrate this concept in vivo, an artificial zinc finger protein (AZP) targeting the replication origin of the Beet severe curly top virus (BSCTV), a model DNA virus, was created. In vitro DNA binding assays indicated that the A...
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We present a novel approach for generating targeted deletions of genomic segments in human and other eukaryotic cells using engineered zinc finger nucleases (ZFNs). We found that ZFNs designed to target two different sites in a human chromosome could introduce two concurrent DNA double-strand breaks (DSBs) in the chromosome and give rise to targeted deletions of the genomic segment between the ...
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Genome engineering through homologous recombination (HR) is a powerful instrument for studying biological pathways or creating treatment options for genetic disorders. In mammalian cells HR is rare but the creation of targeted DNA double-strand breaks stimulates HR significantly. Here, we present a method to generate, evaluate, and optimize rationally designed endonucleases that promote HR. The...
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Agricultural biotechnology is limited by the inefficiency and unpredictability of conventional random mutagenesis and transgenesis. We have developed a broadly applicable, versatile alternative: the use of designed zinc-finger nucleases (ZFNs) that induce double-stranded breaks (DSB) at their target locus. We show that ZFNs designed to cleave an endogenous maize gene disrupt their target via DS...
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Homologous recombination-based gene targeting using Mus musculus embryonic stem cells has greatly impacted biomedical research. This study presents a powerful new technology for more efficient and less time-consuming gene targeting in mice using embryonic injection of zinc-finger nucleases (ZFNs), which generate site-specific double strand breaks, leading to insertions or deletions via DNA repa...
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ژورنال
عنوان ژورنال: Nucleic Acids Symposium Series
سال: 2008
ISSN: 0261-3166,1746-8272
DOI: 10.1093/nass/nrn094